The biotechnological production of pharmaceuticals and biocommodities relies on enzymes. Indeed, the use of multi-enzymatic processes is considered a promising biomanufacturing platform but with several challenges that need to be solved:
- cross-reactivity found in enzyme cascades involving two or more enzymes competing for the same substrate.
- enzyme inactivation or inhibition at unsuitable temperature when using combination of meso and thermophile enzymes.
- finding optimal reaction conditions without negatively impacting on the stability of products and cofactors.